Article
Development of homogeneous immunoassays based on protein fragment complementation.
Biochemical and biophysical research communications - 23 May 2008
de las Heras Rachel, Fry Scott R, Li Jun, Arel Ece, Kachab Edward H, Hazell Stuart L, Huang Chang-Yi
Abstract excerpt
We demonstrate a functional in vitro proof-of-principle homogeneous assay capable of detecting small (<1kDa) to large (150kDa) analytes using TEM-1 beta-lactamase protein fragment complementation. In the assays reported here, complementary components are added together in the presence of analyte and substrate resulting in colorimetric detection within 10-min. We demonstrate the use of functional mutations leading...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.