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Article

Directed evolution of the DnaK chaperone: mutations in the lid domain result in enhanced chaperone activity.

Journal of molecular biology - 28 May 2010

Aponte Raphael A, Zimmermann Sabine, Reinstein Jochen

Abstract excerpt

We improved the DnaK molecular chaperone system for increased folding efficiency towards two target proteins, by using a multi-parameter screening procedure. First, we used a folding-deficient C-terminal truncated chloramphenicol acetyl transferase (CAT_Cd9) to obtain tunable selective pressure for enhanced DnaK chaperon function in vivo. Second, we screened selected clones in vitro for CAT_Cd9 activity after...

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Identifiers

PMID: 20381501
DOI: 10.1016/j.jmb.2010.03.060

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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