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Article

PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high‐throughput sequencing

27 Jan 2021

Abstract excerpt

Abstract The reconstructed in vitro translation system known as the PURE system has been used in a variety of cell‐free experiments such as the expression of native and de novo proteins as well as various display methods to select for functional polypeptides. We developed a refined PURE‐based display method for the preparation of stable messenger RNA (mRNA) and complementary DNA (cDNA)‐peptide conjugates and...

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PMID: 33501662
DOI: 10.1002/bit.27696

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