Article
Efficient discovery of DNA polymorphisms in natural populations by Ecotilling.
The Plant journal : for cell and molecular biology - 1 Mar 2004
Comai Luca, Young Kim, Till Bradley J, Reynolds Steven H, Greene Elizabeth A, Codomo Christine A, Enns Linda C, Johnson Jessica E, Burtner Chris, Odden Anthony R, Henikoff Steven
Abstract excerpt
We have adapted the mutation detection technology used in Targeting Induced Local Lesions in Genomes (TILLING) to the discovery of polymorphisms in natural populations. The genomic DNA of a queried individual is mixed with a reference DNA and used to amplify a target 1-kbp region of DNA with asym...
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