Article
High-throughput discovery of chloroplast and mitochondrial DNA polymorphisms in Brassicaceae species by ORG-EcoTILLING.
PloS one - 1 Jan 2012
Zeng Chang-Li, Wang Guang-Yong, Wang Jian-Bo, Yan Gui-Xin, Chen Bi-Yun, Xu Kun, Li Jun, Gao Gui-Zhen, Wu Xiao-Ming, Zhao Bo, Liu Lei
Abstract excerpt
BACKGROUND: Information on polymorphic DNA in organelle genomes is essential for evolutionary and ecological studies. However, it is challenging to perform high-throughput investigations of chloroplast and mitochondrial DNA polymorphisms. In recent years, EcoTILLING stands out as one of the most universal, low-cost, and high-throughput reverse genetic methods, and the identification of natural genetic variants...
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