Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation.

Nucleic acids research - 24 Apr 2023

Develtere Ward, Waegneer Evelien, Debray Kevin, De Saeger Jonas, Van Glabeke Sabine, Maere Steven, Ruttink Tom, Jacobs Thomas B

Abstract excerpt

Multiplex amplicon sequencing is a versatile method to identify genetic variation in natural or mutagenized populations through eco-tilling or multiplex CRISPR screens. Such genotyping screens require reliable and specific primer designs, combined with simultaneous gRNA design for CRISPR screens. Unfortunately, current tools are unable to combine multiplex gRNA and primer design in a high-throughput and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Variation

Share to Genome

Share to Genotype

Share to Multiplex Polymerase Chain Reaction

Share to Software

Source

Open in PubMed Open full text

Identifiers

PMID: 36718951
DOI: 10.1093/nar/gkad036
PMCID: PMC10123101

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation.PMID 36718951Read ↗

indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events.Semantic proximity Read ↗

SuperDecode: An integrated toolkit for analyzing mutations induced by genome editing.Semantic proximity Read ↗

Discovery of rare mutations in populations: TILLING by sequencing.Semantic proximity Read ↗

A protocol for TILLING and Ecotilling in plants and animals.Semantic proximity Read ↗

FIND-IT: Ultrafast mining of genome diversitySemantic proximity Read ↗

Thousands of trait-specific KASP markers designed for diverse breeding applications in rice (Oryza sativa).Semantic proximity Read ↗

Degenerate oligonucleotide primer MIG-seq: an effective PCR-based method for high-throughput genotyping.Semantic proximity Read ↗

A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley.Semantic proximity Read ↗

CRISPR screens in plants: approaches, guidelines, and future prospects.Semantic proximity Read ↗

Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles.Semantic proximity Read ↗

Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement.Semantic proximity Read ↗

Methods for the design, implementation, and analysis of illumina infinium™ SNP assays in plants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.