Article
Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species.
Nucleic acids research - 15 Mar 1998
Desmarais E, Lanneluc I, Lagnel J
Abstract excerpt
Direct amplification of length polymorphisms (DALP) uses an arbitrarily primed PCR (AP-PCR) to produce genomic fingerprints and to enable sequencing of DNA polymorphisms in virtually any species. Oligonucleotide pairs were designed to each produce a specific multi-banded pattern and all the fragments thus generated can be directly sequenced with the same two universal M13 sequencing primers. This strategy...
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