Diagnosis of acute promyelocytic leukaemia by RT‐PCR: detection of <i>PML‐RARA</i> and <i>RARA‐PML</i> fusion transcripts

Abstract excerpt

Acute promyelocytic leukaemia (APL; AML M3) is identified by a unique t(15;17) translocation which fuses the PML gene to the retinoic acid receptor alpha gene (RARA). Reverse transcription coupled with the polymerase chain reaction (RT-PCR) has been used to develop a diagnostic test for APL based on the PML-RARA fusion message. Separate PCR assays were designed to amplify either PML-RARA (15q+ derived) or...

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