Use of allele-specific sequencing primers is an efficient alternative to PCR subcloning of low-copy nuclear genes.

Abstract excerpt

Direct Sanger sequencing of polymerase chain reaction (PCR)-amplified nuclear genes leads to polymorphic sequences when allelic variation is present. To overcome this problem, most researchers subclone the PCR products to separate alleles. An alternative is to directly sequence the separate alleles using allele-specific primers. We tested two methods to enhance the specificity of allele-specific primers for use...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Cloning, Molecular