Article
Characterization of polymerase chain reaction amplification of specific alleles.
Analytical biochemistry - 1 Apr 1990
Sarkar G, Cassady J, Bottema C D, Sommer S S
Abstract excerpt
Under certain conditions, polymerase chain reaction (PCR) can be used to differentially amplify one allele over another. To characterize the phenomenon, we have made a series of PCR primers and determined whether differential amplification could be detected after agarose gel electrophoresis. Two allele pairs were examined; one pair represents a transversion and one pair represents a transition. The following...
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