Characterization of polymerase chain reaction amplification of specific alleles.

doi:10.1016/0003-2697(90)90573-r

Back to search

Article

Characterization of polymerase chain reaction amplification of specific alleles.

Analytical biochemistry - 1 Apr 1990

Sarkar G, Cassady J, Bottema C D, Sommer S S

Abstract excerpt

Under certain conditions, polymerase chain reaction (PCR) can be used to differentially amplify one allele over another. To characterize the phenomenon, we have made a series of PCR primers and determined whether differential amplification could be detected after agarose gel electrophoresis. Two...

Topics

Alleles
Animals
Base Sequence
DNA
Male
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Sensitivity and Specificity

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.