Article
Analyses of the effects of Rck2p mutants on Pbs2pDD-induced toxicity in Saccharomyces cerevisiae identify a MAP kinase docking motif, and unexpected functional inactivation due to acidic substitution of T379.
Molecular genetics and genomics : MGG - 1 Mar 2004
Jiang L, Niu S, Clines K L, Burke D J, Sturgill T W
Abstract excerpt
Rck2p is a Ser/Thr kinase that binds to, and is activated by, Hog1p. Expression of the MAP kinase kinase Pbs2pDD from a GAL1-driven plasmid hyperactivates the HOG MAP kinase pathway, and leads to cessation of growth. This toxic effect is reduced by deletion of RCK2. We studied the structural and functional basis for the role of Rck2p in mediating the growth arrest phenotype associated with overexpression of...
Topics
- Alleles
- Amino Acid Motifs
- Amino Acid Sequence
- Binding Sites
- DNA Primers
- Gene Deletion
- Gene Expression Regulation, Fungal
- Immunoblotting
- Mitogen-Activated Protein Kinase Kinases
- Mitogen-Activated Protein Kinases