Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts.

Molecular cell - 16 Jan 2004

Yamano Hiroyuki, Gannon Julian, Mahbubani Hiro, Hunt Tim

Abstract excerpt

Substrates for mitotic proteolysis such as cyclin B have a 9 residue destruction motif, the destruction box (D-box). To identify the receptor that specifically binds the D-box, we used affinity chromatography with immobilized D-box matrices. We find that the APC/C from Xenopus egg extracts binds to the D-box of cyclin B, whereas Fizzy (Cdc20) does not. Mutations in the D-box abolished this interaction. We show...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Extracts
Share to Chromatography, Affinity
Share to Cyclin B

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Glutathione Transferase

Share to Oocytes

Share to Protein Kinases

Share to Surface Plasmon Resonance

Share to Xenopus

Source

Identifiers

PMID: 14731401
DOI: 10.1016/s1097-2765(03)00480-5

Genes

APC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts.PMID 14731401Read ↗

The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae.Semantic proximity Read ↗

Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.Semantic proximity Read ↗

Destruction of Xenopus cyclins A and B2, but not B1, requires binding to p34cdc2.Semantic proximity Read ↗

The destruction box of the cyclin Clb2 binds the anaphase-promoting complex/cyclosome subunit Cdc23.Semantic proximity Read ↗

The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised.Semantic proximity Read ↗

Destruction with a box: substrate recognition by the anaphase-promoting complex.Semantic proximity Read ↗

The D box asserts itself.Semantic proximity Read ↗

The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe.Semantic proximity Read ↗

Oncogenic Adenomatous polyposis coli mutants impair the mitotic checkpoint through direct interaction with Mad2.Semantic proximity Read ↗

APC/Fizzy-Related targets Aurora-A kinase for proteolysis.Semantic proximity Read ↗

Analysis of activator-binding sites on the APC/C supports a cooperative substrate-binding mechanism.Semantic proximity Read ↗

Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.