Article
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
Molecular biology of the cell - 1 Nov 1992
Kobayashi H, Stewart E, Poon R, Adamczewski J P, Gannon J, Hunt T
Abstract excerpt
The binding of cyclin A to p34cdc2 and p32cdk2 and the protein kinase activity of the complexes has been measured by cell-free translation of the corresponding mRNA in extracts of frog eggs, followed by immunoprecipitation. A variety of mutant cyclin A molecules have been constructed and tested i...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- CDC2 Protein Kinase
- CDC2-CDC28 Kinases
- Cells, Cultured
- Cyclin-Dependent Kinase 2
- Cyclin-Dependent Kinases
- Cyclins
- Electrophoresis, Polyacrylamide Gel