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Article

High-fidelity DNA polymerase enhances the sensitivity of a peptide nucleic acid clamp PCR assay for K-ras mutations.

The Journal of molecular diagnostics : JMD - 1 Jul 2008

Gilje Bjørnar, Heikkilä Reino, Oltedal Satu, Tjensvoll Kjersti, Nordgård Oddmund

Abstract excerpt

Sensitive detection of tumor-specific point mutations is of interest in both the early detection of cancer and the monitoring of treatment at a molecular level. Recently, peptide nucleic acid (PNA) clamp real-time PCR has provided a time-sparing and sensitive method for the detection of mutations in the presence of a large excess of wild-type DNA. We present the first report that the sensitivity of PNA clamp PCR...

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Source

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Identifiers

PMID: 18556764
DOI: 10.2353/jmoldx.2008.070183
PMCID: PMC2438201

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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