Article
Transcriptional regulation of human excitatory amino acid transporter 1 (EAAT1): cloning of the EAAT1 promoter and characterization of its basal and inducible activity in human astrocytes.
Journal of neurochemistry - 1 Dec 2003
Kim Seon-Young, Choi So-Young, Chao Wei, Volsky David J
Abstract excerpt
Excitatory amino acid transporter 1 (EAAT1) is one of the two glial glutamate transporters that clear the extracellular glutamate generated during neuronal signal transmission. Here, we cloned and characterized a 2.1-kb promoter region of human EAAT1 and investigated its function in the transcriptional regulation of the EAAT1 gene in human primary astrocytes. The full-length promoter region lacked TATA and CCAAT...
Topics
- 8-Bromo Cyclic Adenosine Monophosphate
- Animals
- Astrocytes
- Base Sequence
- Blotting, Northern
- Cells, Cultured
- Cloning, Molecular
- Cyclic CMP
- DNA-Binding Proteins
- Electrophoretic Mobility Shift Assay