Article
Detection of mutations in the mitogen-activated protein kinase pathway in human melanoma.
Clinical cancer research : an official journal of the American Association for Cancer Research - 15 Dec 2003
Alsina Janivette, Gorsk David H, Germino F Joseph, Shih Weichung, Lu Shou-En, Zhang Zhi-Gang, Yang Jin-Ming, Hait William N, Goydos James S
Abstract excerpt
PURPOSE: Recent studies suggest that activating point mutations in B-RAF may commonly occur in melanoma. We devised a method to detect point mutations in heterogeneous tissues containing both wild-type and mutant B-RAF and N-RAS genes by using site-directed mutagenesis to introduce new restrictions sites in the cDNA sequence when the specific point mutations are present. We used this technique to determine the...
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