Article
Mutation of B-Raf in human choroidal melanoma cells mediates cell proliferation and transformation through the MEK/ERK pathway.
The Journal of biological chemistry - 24 Oct 2003
Calipel Armelle, Lefevre Gaelle, Pouponnot Celio, Mouriaux Frédéric, Eychène Alain, Mascarelli Frédéric
Abstract excerpt
The BRAF gene, encoding a mitogen-activated protein kinase kinase kinase, is mutated in several human cancers, with the highest incidence occurring in cutaneous melanoma. The activating V599E mutation accounted for 80% of all mutations detected in cutaneous melanoma cell lines. Reconstitution experiments have shown that this mutation increases ectopically expressed B-Raf kinase activity and induces NIH3T3 cell...
Topics
- Cell Cycle Proteins
- Cell Division
- Choroid Neoplasms
- Gene Expression Profiling
- Humans
- MAP Kinase Kinase 1
- Melanoma
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase Kinases
- Mitogen-Activated Protein Kinases
- Mutation
- Oncogene Proteins