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Article

Effect of multiple symmetries on the association of R67 DHFR subunits bearing interfacial complementing mutations.

Protein science : a publication of the Protein Society - 1 Jan 2004

Dam Julie, Blondel Arnaud

Abstract excerpt

It was shown previously that complementation could be a powerful mean to probe protein-protein interactions in the normally tetrameric R67 DHFR. Indeed, mixing complementing inactive dimeric mutants produced active heterotetramers. This approach turned a homo-oligomer into a hetero-oligomer and thus allowed the use of combinatorial assays, a subtle analysis of the association forces, and a precise determination...

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Identifiers

PMID: 14691216
DOI: 10.1110/ps.03309504
PMCID: PMC2286515

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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