Article
Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Nov 2011
Yachnin Brahm J, Colin Damien Y, Volpato Jordan P, Ebert Maximilian, Pelletier Joelle N, Berghuis Albert M
Abstract excerpt
Trimethoprim is an antibiotic that targets bacterial dihydrofolate reductase (DHFR). A plasmid-encoded DHFR known as R67 DHFR provides resistance to trimethoprim in bacteria. To better understand the mechanism of this homotetrameric enzyme, a tandem dimer construct was created that linked two monomeric R67 DHFR subunits together and mutated the sequence of residues 66-69 of the first subunit from VQIY to INSF....
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