Germline variable region gene segment derivation of human monoclonal anti-Rh(D) antibodies. Evidence for affinity maturation by somatic hypermutation and repertoire shift.

doi:10.1172/JCI116140

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Article

Germline variable region gene segment derivation of human monoclonal anti-Rh(D) antibodies. Evidence for affinity maturation by somatic hypermutation and repertoire shift.

The Journal of clinical investigation - 1 Dec 1992

Bye J M, Carter C, Cui Y, Gorick B D, Songsivilai S, Winter G, Hughes-Jones N C, Marks J D

Abstract excerpt

To date, there has been no systematic study of the process of affinity maturation of human antibodies. We therefore sequenced the variable region genes (V genes) of 14 human monoclonal antibodies specific for the erythrocyte Rh(D) alloantigen and determined the germline gene segments of origin an...

Topics

Antibodies, Monoclonal
Antibody Affinity
Base Sequence
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Gene Rearrangement, B-Lymphocyte, Light Chain
Genes, Immunoglobulin
Humans
Immunoglobulin G
Immunoglobulin Isotypes
Immunoglobulin Light Chains

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoglobulin M

Immunoglobulin Variable Region

Molecular Sequence Data

Mutation

Oligodeoxyribonucleotides

Rh-Hr Blood-Group System

Sequence Alignment

Source

Open in PubMed Open full text

Identifiers

PMID: 1469099
DOI: 10.1172/JCI116140
PMCID: PMC443405

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1469099-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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