Article
A conserved proline in the last transmembrane segment of Gaa1 is required for glycosylphosphatidylinositol (GPI) recognition by GPI transamidase.
The Journal of biological chemistry - 20 Feb 2004
Vainauskas Saulius, Menon Anant K
Abstract excerpt
Glycosylphosphatidylinositol (GPI)-anchored proteins are synthesized as precursor proteins that are processed in the endoplasmic reticulum by GPI transamidase (GPIT). Human GPIT is a multisubunit membrane-bound protein complex consisting of Gaa1, Gpi8, phosphatidylinositol glycan (PIG)-S, PIG-T, and PIG-U. The enzyme recognizes a C-terminal signal sequence in the proprotein and replaces it with a preformed GPI...
Topics
- Acyltransferases
- Amino Acid Sequence
- Animals
- Conserved Sequence
- DNA, Complementary
- Flow Cytometry
- HeLa Cells
- Hexosaminidases
- Humans
- Mannose
- Membrane Glycoproteins
- Mice
- Molecular Sequence Data
- Mutation
- Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase