Article
Study of MICA alleles in 201 African Americans by multiplexed single nucleotide extension (MSNE) typing.
Human immunology - 1 Jan 2003
Zhang Yanzheng, Han Mei, Vorhaben Robert, Giang Chris, Lavingia Bhavna, Stastny Peter
Abstract excerpt
We have developed a method for major histocompatibility complex class I chain-related gene A (MICA) genotyping using multiplexed single nucleotide extension (MSNE) and flow cytometric analysis of an array of fluorescent microspheres. This technique employs a polymerase chain reaction-derived target DNA containing all the polymorphic sites of MICA, synthetic complementary primers, biotinylated dideoxynucleotide...
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