Article
Cloning and preliminary characterization of srf-2020 and srf-801, the recF partial suppressor mutations which map in recA of Escherichia coli K-12.
Biochimie - 1 Jan 2000
Wang T C, Madiraju M V, Templin A, Clark A J
Abstract excerpt
We have located the single nucleotide changes suffered in recA sequence of 2 recF partial suppressor mutations: srf-2020 at codon 121 and srf-801 at codon 257. srf-2020 changes codon 121 from threonine (ACC) to isoleucine (ATC). srf-801 changes codon 257 from glutamine (CAG) to proline (CCG). Consequently these mutations were renamed recA2020 and recA801 respectively. Preliminary characterization of recA2020...
Topics
- Alleles
- Bacterial Proteins
- Chromosome Mapping
- Cloning, Molecular
- Codon
- DNA Repair
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genes, Suppressor
