Article
A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis.
The Journal of biological chemistry - 20 Feb 2004
De Simone Giuseppina, Mandrich Luigi, Menchise Valeria, Giordano Valeria, Febbraio Ferdinando, Rossi Mosè, Pedone Carlo, Manco Giuseppe
Abstract excerpt
The reaction mechanism of the esterase 2 (EST2) from Alicyclobacillus acidocaldarius was studied at the kinetic and structural level to shed light on the mechanism of activity and substrate specificity increase previously observed in its double mutant M211S/R215L. In particular, the values of kinetic constants (k1, k(-1), k2, and k3) along with activation energies (E1, E(-1), E2, and E3) were measured for wild...
Topics
- Alcohols
- Bacteria
- Binding Sites
- Caproates
- Carboxylic Ester Hydrolases
- Catalysis
- Crystallography, X-Ray
- Dose-Response Relationship, Drug
- Kinetics
- Laurates
- Models, Chemical
- Models, Molecular
- Mutation