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Article

A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis.

The Journal of biological chemistry - 20 Feb 2004

De Simone Giuseppina, Mandrich Luigi, Menchise Valeria, Giordano Valeria, Febbraio Ferdinando, Rossi Mosè, Pedone Carlo, Manco Giuseppe

Abstract excerpt

The reaction mechanism of the esterase 2 (EST2) from Alicyclobacillus acidocaldarius was studied at the kinetic and structural level to shed light on the mechanism of activity and substrate specificity increase previously observed in its double mutant M211S/R215L. In particular, the values of kinetic constants (k1, k(-1), k2, and k3) along with activation energies (E1, E(-1), E2, and E3) were measured for wild...

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Identifiers

PMID: 14617621
DOI: 10.1074/jbc.M307738200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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