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Article

Improving the thermal stability of trans-epoxysuccinate hydrolase.

Journal of Zhejiang University. Science. B - 22 Dec 2025

Bao Wenna, Yao Jinfeng, Pan Haifeng, Zhu Ronglin, Li Xinying, Liao Hongxiu

Abstract excerpt

This study used molecular dynamics simulations, B-factor analysis, and saturation mutagenesis screening to enhance the thermal stability of the trans-epoxysuccinate hydrolase (TESH) derived from Pseudomonas koreensis. Eleven mutants that influence this characteristic were selected, yielding four mutants with improved activity. Among them, mutants A142C and S178Q exhibited lower Michaelis constant (Km) values, and...

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Identifiers

PMID: 41601369
DOI: 10.1631/jzus.B2500069
PMCID: PMC12848554

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