Article
Role of the N-terminal region for the conformational stability of esterase 2 from Alicyclobacillus acidocaldarius.
Biophysical chemistry - 1 Apr 2007
Foglia Fabrizia, Mandrich Luigi, Pezzullo Margherita, Graziano Giuseppe, Barone Guido, Rossi Mosè, Manco Giuseppe, Del Vecchio Pompea
Abstract excerpt
In order to clarify the role played by the N-terminal region for the conformational stability of the thermophilic esterase 2 (EST2) from Alicyclobacillus acidocaldarius, two mutant forms have been investigated: a variant obtained by deleting the first 35 residues at the N-terminus (EST2-36del), and a variant obtained by mutating Lys102 to Gln (K102Q) to perturb the N-terminus by destroying the salt bridge...
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