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Article

Patch clamp analysis of a H+ pump heterologously expressed in giant yeast vacuoles.

Journal of biochemistry - 1 Oct 2003

Nakanishi Yoichi, Yabe Isamu, Maeshima Masayoshi

Abstract excerpt

Despite the usefulness of the patch-clamp technique, its application to ion pumps and transporters in biomembranes is limited. We developed a novel method for determining the activity of a proton-pumping pyrophosphatase (H(+)-PPase) made of a single protein. We heterologously highly expressed the enzyme in Saccharomyces cerevisiae, prepared giant vacuoles from the cells, and measured a PPi-dependent electrical...

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PMID: 14607990
DOI: 10.1093/jb/mvg184

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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