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Article

Probing essential water in yeast pyrophosphatase by directed mutagenesis and fluoride inhibition measurements.

The Journal of biological chemistry - 5 Jan 2001

Pohjanjoki P, Fabrichniy I P, Kasho V N, Cooperman B S, Goldman A, Baykov A A, Lahti R

Abstract excerpt

The pattern of yeast pyrophosphatase (Y-PPase) inhibition by fluoride suggests that it replaces active site Mg(2+)-bound nucleophilic water, for which two different locations were proposed previously. To localize the bound fluoride, we investigate here the effects of mutating Tyr(93) and five dic...

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PMID: 11031269
DOI: 10.1074/jbc.M007360200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-11031269-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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