Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles.

doi:10.1016/j.bbamem.2005.03.011

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Article

Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles.

Biochimica et biophysica acta - 30 Jun 2005

Ohgaki Ryuichi, Nakamura Norihiro, Mitsui Keiji, Kanazawa Hiroshi

Abstract excerpt

The Saccharomyces cerevisiae Nha1p, a plasma membrane protein belonging to the monovalent cation/proton antiporter family, plays a key role in the salt tolerance and pH regulation of cells. We examined the molecular function of Nha1p by using secretory vesicles isolated from a temperature sensitive secretory mutant, sec4-2, in vitro. The isolated secretory vesicles contained newly synthesized Nha1p en route to...

Topics

Aminoacridines
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Catalysis
Cation Transport Proteins
Cations
Cell Membrane
Electrophoresis, Polyacrylamide Gel
Green Fluorescent Proteins
Hydrogen-Ion Concentration
Immunoblotting
Ions
Kinetics
Membrane Proteins

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microscopy, Fluorescence

Mutation

Open Reading Frames

Plasmids

Protein Structure, Tertiary

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Open in PubMed

Identifiers

PMID: 15950597
DOI: 10.1016/j.bbamem.2005.03.011

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