Article
Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles.
Biochimica et biophysica acta - 30 Jun 2005
Ohgaki Ryuichi, Nakamura Norihiro, Mitsui Keiji, Kanazawa Hiroshi
Abstract excerpt
The Saccharomyces cerevisiae Nha1p, a plasma membrane protein belonging to the monovalent cation/proton antiporter family, plays a key role in the salt tolerance and pH regulation of cells. We examined the molecular function of Nha1p by using secretory vesicles isolated from a temperature sensitive secretory mutant, sec4-2, in vitro. The isolated secretory vesicles contained newly synthesized Nha1p en route to...
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