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Article

Oligomerization of H(+)-pyrophosphatase and its structural and functional consequences.

Biochimica et biophysica acta - 15 Jul 2005

Mimura Hisatoshi, Nakanishi Yoichi, Maeshima Masayoshi

Abstract excerpt

The H(+)-pyrophosphatase (H(+)-PPase) consists of a single polypeptide, containing 16 or 17 transmembrane domains. To determine the higher order oligomeric state of Streptomyces coelicolor H(+)-PPase, we constructed a series of cysteine substitution mutants and expressed them in Escherichia coli. Firstly, we analyzed the formation of disulfide bonds, promoted by copper, in mutants with single cysteine...

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PMID: 15953583
DOI: 10.1016/j.bbabio.2005.05.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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