Article
Expression of HCV genotype-4 core antigen in prokaryotic E. coli system for diagnosis of HCV infection in Egypt.
Protein expression and purification - 1 Dec 2021
Saleh Eman M, Gouda Abdullah E, Medhat Amina M, Ahmed Hend O, Shemis Mohamed A
Abstract excerpt
BACKGROUND: Egypt has a high prevalence of hepatitis C virus (HCV) infection with 92.5% of genotype-4. AIM: This study aimed to clone and express the core gene of HCV genotype-4 for using it to develop a highly sensitive, specific, and cost-effective diagnostic assay for detecting HCV infection. METHODS: Using synthetic HCV genotype-4 core gene, pET15b as E. coli expression vector, and 1 mM lactose as inducer,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.