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Article

Novel enzyme-linked minisequence assay for genotypic analysis of human immunodeficiency virus type 1 drug resistance.

Journal of clinical microbiology - 1 Nov 2003

Sugiura Wataru, Shimada Kazunori, Matsuda Masakazu, Chiba Tomoko, Myint Lay, Okano Aiko, Yamada Kaneo

Abstract excerpt

We constructed a novel tool for genotypic analysis of human immunodeficiency virus type 1 (HIV-1) drug resistance by using an enzyme-linked minisequence assay (ELMA). ELMA is a combination of hybridization and a 1-base extension reaction, and we designed the assay to detect five mutations conferring nucleoside analogue resistance (M41L, D67N, K70R, T215Y, and M184V) and six mutations conferring protease inhibitor...

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Identifiers

PMID: 14605126
DOI: 10.1128/JCM.41.11.4971-4979.2003
PMCID: PMC262484

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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