Article
Genetic analysis of the tetA(C) gene on plasmid pBR322.
Journal of bacteriology - 1 Dec 1992
McNicholas P, Chopra I, Rothstein D M
Abstract excerpt
The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetr...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- DNA Mutational Analysis
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Hydrogen-Ion Concentration
- Kinetics
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Potassium
- Protein Structure, Secondary
- R Factors
- Repressor Proteins
- Tetracycline Resistance