Article
Purification and characterization of Tet(M), a protein that renders ribosomes resistant to tetracycline.
The Journal of biological chemistry - 15 Feb 1991
Burdett V
Abstract excerpt
The tet(M) tetracycline resistance gene has been found in a wide variety of clinically important bacteria. It has been shown previously (Burdett, V. (1986) J. Bacteriol. 165, 564-569) that the tet(M) gene product mediates resistance at the level of protein synthesis as judged by in vitro assay. Using this assay, large amounts of protein were purified from an Escherichia coli overproducer expressing the gene under...
Topics
- Amino Acid Sequence
- Amino Acids
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- GTP Phosphohydrolase-Linked Elongation Factors
- Genes, Bacterial
- Hot Temperature
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Peptide Elongation Factor G