Article
Metabolic characteristics of an isocitrate dehydrogenase defective derivative of escherichia coli BL21(DE3).
Biotechnology and bioengineering - 20 Dec 2003
Aoshima Miho, Ishii Masaharu, Yamagishi Akihiko, Oshima Tairo, Igarashi Yasuo
Abstract excerpt
A 7.8 kb fragment containing isocitrate dehydrogenase (ICDH) gene and its flanking regions was cloned from Escherichia coli BL21(DE3) and sequenced. Unlike the case of the K-12 strain, the e14 element was not found. The nucleotide divergence between these two strains was about 2%. Using the cloned fragment, ICDH defective mutant strain, MA1935, was generated from BL21(DE3). Although MA1935 accumulated citrate,...
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