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Continuous culture and proteomic analysis of Escherichia coli DH5α and its acetate-tolerant mutant DA19 under conditions of nitrogen source limitation.

Bioprocess and biosystems engineering - 1 Feb 2011

Zhang Xiaoyun, Zhang Yanjun, Li Zhimin, Xia Yuelan, Ye Qin

Abstract excerpt

Escherichia coli DH5α grows poorly in a chemically defined medium, but its acetate-tolerant mutant, DA19, grows fast and produces less acetate. To investigate the differences in metabolism between the two strains, the samples of continuous cultures at steady state were analyzed by two-dimensional SDS-PAGE (2-D SDS-PAGE) and peptide mass fingerprinting (PMF). The results indicated that in DA19, expression of gnd,...

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PMID: 20700606
DOI: 10.1007/s00449-010-0459-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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