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Article

Blending wine yeast phenotypes with the aid of CRISPR DNA editing technologies.

International journal of food microbiology - 2 Jul 2020

van Wyk Niël, Kroukamp Heinrich, Espinosa Monica I, von Wallbrunn Christian, Wendland Jürgen, Pretorius Isak S

Abstract excerpt

In recent years, CRISPR/Cas9-based genetic editing has become a mainstay in many laboratories including manipulations done with yeast. We utilized this technique to generate a self-cloned wine yeast strain that overexpresses two genes of oenological relevance i.e. the glycerol-3-phosphate dehydrogenase 1 (GPD1) and the alcohol acetyltransferase 1 (ATF1) directly implicated in glycerol and acetate ester production...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32371236
DOI: 10.1016/j.ijfoodmicro.2020.108615

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