Article
Inducible expression system and marker-linked mutagenesis approach for functional genomics of Haemophilus influenzae.
Gene - 16 Oct 2003
Wong Sandy M, Akerley Brian J
Abstract excerpt
Complete bacterial genomic DNA sequences have allowed application of genome-scale strategies to identify essential genes. Efficient generation of conditional loss of function mutations provides a means of characterization of this class of genes. Promoter systems conferring tight regulation are particularly applicable to generating such mutations and we sought to apply this approach for the analysis of an...
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