Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch hydrolysis.

doi:10.2174/0929866033478744

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Article

Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch hydrolysis.

Protein and peptide letters - 1 Oct 2003

Wong Dominic W S, Batt Sarah B, Lee Charles C, Robertson George H

Abstract excerpt

High-throughput screening for high-activity barley alpha-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed colorimetric and chemiluminescent detection systems that enable direct and rapid screening of activities on raw starch substrate. Active clones could be separated...

Topics

Base Sequence
Cloning, Molecular
Enzyme Activation
Gene Library
Genetic Testing
Glycerol
Hydrolysis
Luminescent Measurements
Molecular Sequence Data
Mutation
Recombinant Proteins
Saccharomyces cerevisiae
Starch
Substrate Specificity
alpha-Amylases

Source

Open in PubMed

Identifiers

PMID: 14561134
DOI: 10.2174/0929866033478744

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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