Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A nonradioactive, high throughput assay for chitin synthase activity.

Analytical biochemistry - 1 Jun 2002

Lucero Héctor A, Kuranda Michael J, Bulik Dorota A

Abstract excerpt

Wheat germ agglutinin (WGA) binds with high affinity and specificity to several sites on chitin polymers. Based on these properties we have modified and adapted a previously patented (U.S. patent 5,888,757) nonradioactive, high throughput screening assay for antimicrobial agents, making it suitable as a quantitative enzymatic assay for the activity of individual chitin synthase isozymes in yeast. The procedure...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chitin Synthase
Share to Cobalt
Share to Colorimetry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Dithiothreitol

Share to Horseradish Peroxidase

Share to Nickel

Share to Plant Lectins

Share to Sodium Dodecyl Sulfate

Source

Identifiers

PMID: 12018950
DOI: 10.1006/abio.2002.5594

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A nonradioactive, high throughput assay for chitin synthase activity.PMID 12018950Read ↗

A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A.Semantic proximity Read ↗

Molecular and genetic control of chitin biosynthesis in fungi.Semantic proximity Read ↗

Disruption of two genes for chitin synthase in the phytopathogenic fungus Ustilago maydis.Semantic proximity Read ↗

2-Acylamido analogues of N-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2.Semantic proximity Read ↗

Compromised chitin synthesis in lager yeast affects its Congo red resistance and release of mannoproteins from the cells.Semantic proximity Read ↗

Mutation of active site residues in the chitin-binding domain ChBDChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: use of a green fluorescent protein binding assay.Semantic proximity Read ↗

A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts.Semantic proximity Read ↗

WdChs2p, a class I chitin synthase, together with WdChs3p (class III) contributes to virulence in Wangiella (Exophiala) dermatitidis.Semantic proximity Read ↗

Functional differentiation of chitin synthases in Yarrowia lipolytica.Semantic proximity Read ↗

A diverse range of bacterial and eukaryotic chitinases hydrolyzes the LacNAc (Galβ1-4GlcNAc) and LacdiNAc (GalNAcβ1-4GlcNAc) motifs found on vertebrate and insect cells.Semantic proximity Read ↗

Chitin synthesis in Saccharomyces cerevisiae in response to supplementation of growth medium with glucosamine and cell wall stress.Semantic proximity Read ↗

The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.