Article
An Activity-Staining Method on Filtration Paper Enables High-Throughput Screening of Temperature-Sensitive and Inactive Mutations of Rice α-Amylase for Improvement of Rice Grain Quality.
Plant & cell physiology - 1 Apr 2017
Yamakawa Hiromoto, Hirai-Kimura Rieko, Nakata Yuriko, Nakata Masaru, Kuroda Masaharu, Yamaguchi Takeshi
Abstract excerpt
α-Amylase is a starch-hydrolyzing enzyme (EC 3.2.1.1) indispensable for germination of cereal seeds, but it is also expressed during the ripening stage. Previous studies demonstrated that the enzyme is activated in developing rice seeds under extremely hot weather and triggers a loss of grain quality by hindering the accumulation of storage starch in the endosperm. Since inactive or, preferably, heat-labile...
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