Article
Pathogen genotyping in polyclonal infections: application of a fluorogenic polymerase-chain-reaction assay in malaria.
The Journal of infectious diseases - 15 Oct 2003
Decuypere Saskia, Elinck Ellen, Van Overmeir Chantal, Talisuna Ambrose O, D'Alessandro Umberto, Dujardin Jean-Claude
Abstract excerpt
Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1). how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2). how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy...
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