Article
Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.
Antimicrobial agents and chemotherapy - 1 Dec 2000
Durand R, Eslahpazire J, Jafari S, Delabre J F, Marmorat-Khuong A, di Piazza J P, Le Bras J
Abstract excerpt
A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.
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