Article
Real-time quantitative PCR for analysis of genetically mixed infections of malaria parasites: technique validation and applications.
Molecular and biochemical parasitology - 1 Oct 2003
Cheesman Sandra J, de Roode Jacobus C, Read Andrew F, Carter Richard
Abstract excerpt
A technique that can distinguish and quantify genetically different malaria parasite clones in a mixed infection reliably and with speed and accuracy would be very useful for researchers. Many current methods of genotyping and quantification fall down on a number of aspects relating to their ease of use, sensitivity, cost, reproducibility and, not least, accuracy. Here we report the development and validation of...
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