Article
Mutation analysis of the Cys-X2-Cys-X19-Cys-X2-Cys motif in the beta subunit of eukaryotic translation initiation factor 2.
Gene expression - 1 Jan 1992
Castilho-Valavicius B, Thompson G M, Donahue T F
Abstract excerpt
Recessive lethal mutations in the beta subunit of eIF-2 that restore HIS4 expression in the absence of an AUG start codon were isolated from diploid Saccharomyces cerevisiae strains. DNA sequence analysis of these alleles and of eIF-2 beta suppressor alleles isolated from haploid strains, identif...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Eukaryotic Initiation Factor-2
- Fungal Proteins
- Genes, Fungal
- Genes, Lethal
- Genes, Suppressor
- Humans
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Site-Directed
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Sequence Alignment
- Sequence Homology, Amino Acid
- Zinc Fingers