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Mutations which enhance the replication of dengue virus type 4 and an antigenic chimeric dengue virus type 2/4 vaccine candidate in Vero cells.

Vaccine - 1 Oct 2003

Blaney Joseph E, Manipon Gracielle G, Firestone Cai Yen, Johnson Daniel H, Hanson Christopher T, Murphy Brian R, Whitehead Stephen S

Abstract excerpt

Mutations which increase the replication of dengue viruses in cell culture would greatly facilitate the manufacture of both a live attenuated or inactivated dengue virus vaccine. We have identified eight missense mutations in dengue virus type 4 (DEN4) that increase the plaque size and kinetics of replication of recombinant DEN4 virus in Vero cells. DEN4 viruses bearing these Vero cell adaptation mutations were...

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Identifiers

PMID: 14505914
DOI: 10.1016/s0264-410x(03)00487-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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