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Article

Mutations which enhance the replication of dengue virus type 4 and an antigenic chimeric dengue virus type 2/4 vaccine candidate in Vero cells.

Vaccine - 1 Oct 2003

Blaney Joseph E, Manipon Gracielle G, Firestone Cai Yen, Johnson Daniel H, Hanson Christopher T, Murphy Brian R, Whitehead Stephen S

Abstract excerpt

Mutations which increase the replication of dengue viruses in cell culture would greatly facilitate the manufacture of both a live attenuated or inactivated dengue virus vaccine. We have identified eight missense mutations in dengue virus type 4 (DEN4) that increase the plaque size and kinetics of replication of recombinant DEN4 virus in Vero cells. DEN4 viruses bearing these Vero cell adaptation mutations were...

Topics

Animals
Animals, Suckling
Antigens, Viral
Brain
Chlorocebus aethiops
Dengue Virus
Kinetics
Mice
Mutation
Phenotype
Recombinant Fusion Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Vaccines, Attenuated

Vero Cells

Viral Plaque Assay

Viral Vaccines

Virus Replication

Source

Identifiers

PMID: 14505914
DOI: 10.1016/s0264-410x(03)00487-0

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