Article
Genetic basis of attenuation of dengue virus type 4 small plaque mutants with restricted replication in suckling mice and in SCID mice transplanted with human liver cells.
Virology - 15 Aug 2002
Blaney Joseph E, Johnson Daniel H, Manipon Gracielle G, Firestone Cai-Yen, Hanson Christopher T, Murphy Brian R, Whitehead Stephen S
Abstract excerpt
Mutations that restrict replication of dengue virus have been sought for the generation of recombinant live-attenuated dengue virus vaccines. Dengue virus type 4 (DEN4) was previously grown in Vero cells in the presence of 5-fluorouracil, and the characterization of 1248 mutagenized, Vero cell passaged clones identified 20 temperature-sensitive (ts) mutant viruses that were attenuated (att) in suckling mouse...
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