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Article

Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5' noncoding region.

Journal of biomedicine & biotechnology - 1 Jan 2010

Leardkamolkarn Vijittra, Sirigulpanit Wipawan, Kinney Richard M

Abstract excerpt

Variants of wild-type dengue serotype 2 (DEN-2) virus containing nucleotide substitutions at positions 14, 15, or 57 in the 5' NCR were constructed by PCR-mediated site-directed mutagenesis. All three viruses containing a single point substitution demonstrated attenuation phenotype as evidenced by decreases replication and plaque size in cell culture assay. All three variants were less neurovirulent in newborn...

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Identifiers

PMID: 20339476
DOI: 10.1155/2010/934694
PMCID: PMC2843905

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