Article
A sensitive dual-fluorescence reporter system enables positive selection of ras suppressors by suppression of ras-induced apoptosis.
Cancer gene therapy - 1 Oct 2003
Dolnikov Alla, Shen Sylvie, Millington Michelle, Passioura Toby, Pedler Michelle, Rasko John Edward Joshua, Symonds Geoff
Abstract excerpt
We have developed a novel dual-fluorescence reporter system incorporating green (GFP) and red (RFP) fluorescent proteins to monitor expression of the N-ras(m) gene and an N-ras(m) suppressor, respectively. Retroviral vectors were produced in which human N-ras(m) (codon 13 mutation) was coexpressed with GFP, and a ribozyme specifically targeting N-ras(m) was coexpressed with RFP. N-Ras(m) suppression was monitored...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.