Article
Homogeneous real-time detection of single-nucleotide polymorphisms by strand displacement amplification on the BD ProbeTec ET system.
Clinical chemistry - 1 Oct 2003
Wang Sha-Sha, Thornton Keith, Kuhn Andrew M, Nadeau James G, Hellyer Tobin J
Abstract excerpt
BACKGROUND: The BD ProbeTec ET System is based on isothermal strand displacement amplification (SDA) of target nucleic acid coupled with homogeneous real-time detection using fluorescent probes. We have developed a novel, rapid method using this platform that incorporates a universal detection format for identification of single-nucleotide polymorphisms (SNPs) and other genotypic variations. METHOD: The system...
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